|
|
| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
12/07/2022 |
Actualizado : |
12/07/2022 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
Complemento del título : |
Chapter 16. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
ISBN : |
Online: 978-1-0716-2301-5 |
DOI : |
10.1007/978-1-0716-2301-5_16 |
Idioma : |
Inglés |
Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
Asunto categoría : |
-- |
Marc : |
LEADER 02176naa a2200349 a 4500 001 1063414 005 2022-07-12 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1007/978-1-0716-2301-5_16$2DOI 100 1 $aKALDS, P. 245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource] 260 $c2022 520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. 650 $aCABRAS 650 $aOVEJA 650 $aPRODUCCION DE CARNE 653 $aCRISPR/Cas9 653 $aGenome editing 653 $aGoats 653 $aKnockout 653 $aMeat production 653 $aMicroinjection 653 $aMSTN 653 $aSheep 653 $aSmall ruminants 700 1 $aCRISPO, M. 700 1 $aTESSON, L. 700 1 $aANEGÓN, I. 700 1 $aCHEN KEY, Y. 700 1 $aWANG, X. 700 1 $aMENCHACA, A. 773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registros recuperados : 28 | |
1. | | MENCHACA, A. Assisted Reproductive Technologies (ART) and genome editing to support a sustainable livestock. Thematic Section: 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE). Animal Reproduction, 2023, Volume 20, Issue 2, e20230074. http://dx.doi.org/10.1590/1984-3143-AR2023-0074 -- OPEN ACCESS. Article history: Received May 26, 2023; Accepted July 21, 2023. -- Document type: Article Gold Open Access, Green Open Access. -- Correspondence: Menchaca, A.; Plataforma de Salud Animal, Instituto Nacional de Investigación...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
| |
3. | | MENCHACA, A. Sustainable food production: The contribution of genome editing in livestock. Sustainability (Switzerland), June 2021, volume 13, Issue 122, article 6788. OPEN ACCESS. Doi: https://doi.org/10.3390/su13126788 Article history: Received 16 March 2021; Revised 31 May 2021; Accepted 1 June 2021; Published 16 June 2021.
Academic Editor: Gerhart U. Ryffel.
This article belongs to the Special Issue Genome Editing in Animal System to Support...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
| |
4. | | BÓ, G.A.; MENCHACA, A. Prohibition of hormones in animal reproduction: what to expect and what to do?. Thematic Section: 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE). Animal Reproduction, 2023, Volume 20, Issue 2, e20230067. https://doi.org/10.1590/1984-3143-AR2023-0067 -- OPEN ACCESS. Article history: Received May 15, 2023; Accepted: July 18, 2023. -- Financial support: GAB received funding for this research from Fondo Nacional de Ciencia y Tecnología (FONCYT PICT 2017-4550), Instituto de Investigación Universidad...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
| |
8. | | GARCÍA PINTOS, C.; RIET-CORREA, F.; MENCHACA, A. Effect of foot-and-mouth disease vaccine on pregnancy failure in beef cows. Frontiers in Veterinary Science, November 2021, volume 8, Article number 761304. Open Access. doi: https://doi.org/10.3389/fvets.2021.761304 Article history: Received 19 August 2021; Accepted 18 October 2021; Published 12 November 2021.
Corresponding author: Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay;...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
| |
12. | | KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. Chapter 16. Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16Tipo: Capítulo en Libro Técnico-Científico |
Biblioteca(s): INIA La Estanzuela. |
| |
17. | | NOVAS, R.; BASIKA, T.; WILLIAMSON, M. E.; FRESIA, P.; MENCHACA, A.; SCOTT, M. J. Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. Insect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS. Article history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
| |
19. | | DOS SANTOS-NETO, P.C.; CUADRO, F.; SOUZA-NEVES, M.; CRISPO, M.; MENCHACA, A. Refinements in embryo manipulation applied to CRISPR technology in livestock. Theriogenology. 2023, Volume 208, Pages 142-148. https://doi.org/10.1016/j.theriogenology.2023.05.028 Article history: Received 14 April 2023; Received in revised form 29 May 2023; Accepted 29 May 2023; Available online 9 June 2023. -- Correspondence author: Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
| |
20. | | CUADRO, F.; DE BRUN, V.; BROCHADO, C.; SOUZA, M.; GARCÍA PINTOS, C.; MENEZES, C.; GASTAL, G.D.A.; MENCHACA, A. Gene expression patterns in uterus and oviduct during the preovulatory period in ewes. In: 19th International Congress on Animal Reproduction (ICAR), Bologna (Italy), 26-30 June 2022. p.185.Biblioteca(s): INIA La Estanzuela. |
| |
Registros recuperados : 28 | |
|
Expresión de búsqueda válido. Check! |
|
|